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Schedule Event local time

18
Apr 2016
  • 07:30 - 08:00
    Welcome and introduction to the course

    Welcome to the course

  • 08:00 - 10:00
    What is epigenetics and how can epigenetics be studied?

    Dr. Ann Van Soom. Univ of Ghent, Belgium

  • 10:00 - 12:00
    Epigenetics mechanisms in reproductive biology

    Dr Sebastian Cánovas. Dept of Physiology. University of Murcia

  • 13:30 - 15:30
    DNA methylation profiling in sigles cells: Challenges and opportunities.

    Dr. Gavin Kelsey. The Babraham Institute/ University of Cambridge

19
Apr 2016
  • 07:00 - 08:00
    Lab session. Step I (restricted to COST-student)

    Cell lysis and bisulfite treatment.

  • 08:00 - 10:00
    Understanding how DNA methylation is patterned in the gametes

    Dr. Gavin Kelsey. The Babraham Institute/ University of Cambridge

  • 10:00 - 11:30
    Lab session. Step II. (restricted to COST-student)

    Bisulfite treatment. First strand synthesis.

  • 13:30 - 14:30
    Lab session. Step III (restricted to COST-student)

    Exo I treatment

20
Apr 2016
  • 07:00 - 08:00
    Lab session. Step IV (restricted to COST-student)

    Reaction clean up: beads purification

  • 08:00 - 09:00
    Epigenetics control in embryonic cell lines.

    Dr. Susana Chuva Sousa Lopes. Dept of Anatomy and Embryology, Leiden University Medical Center

  • 09:00 - 10:00
    Epigenetics re-programming through the germ cell development and gametogenesis.

    Dr. Susana Chuva Sousa Lopes. Dept of Anatomy and Embryology, Leiden University Medical Center

  • 10:00 - 12:00
    Impact of DNA methylation in human testicular tumors of germinal cells

    Dr. Sebastian Cánovas. Dept of Physiology, University of Murcia

  • 13:30 - 16:30
    Lab session. Step V (restricted to COST-student)

    Biotin capture, second strand synthesis and PCR amplification.

21
Apr 2016
  • 07:00 - 08:30
    Lab session. Step VI (restricted to COST-student)

    Library purification

  • 08:30 - 09:30
    Impact of epigenetics in embryonic development and adaptation

    Dr. Alfonso Gutierrez Adan. Dept of Animal Reproduction INIA-Madrid

  • 10:30 - 12:00
    The theory of bisulphite methylation data processing and analysis

    Dr. Simon Andrews. The Babraham Institute

     
  • 13:30 - 15:30
    Lab session. (restricted to COST-student)

    Differential methylation analysis from bisulphite data.

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