Programa En hora local del evento
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07:30 - 08:00Welcome and introduction to the course
Welcome to the course
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08:00 - 10:00What is epigenetics and how can epigenetics be studied?
Dr. Ann Van Soom. Univ of Ghent, Belgium
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10:00 - 12:00Epigenetics mechanisms in reproductive biology
Dr Sebastian Cánovas. Dept of Physiology. University of Murcia
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13:30 - 15:30DNA methylation profiling in sigles cells: Challenges and opportunities.
Dr. Gavin Kelsey. The Babraham Institute/ University of Cambridge
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07:00 - 08:00Lab session. Step I (restricted to COST-student)
Cell lysis and bisulfite treatment.
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08:00 - 10:00Understanding how DNA methylation is patterned in the gametes
Dr. Gavin Kelsey. The Babraham Institute/ University of Cambridge
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10:00 - 11:30Lab session. Step II. (restricted to COST-student)
Bisulfite treatment. First strand synthesis.
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13:30 - 14:30Lab session. Step III (restricted to COST-student)
Exo I treatment
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07:00 - 08:00Lab session. Step IV (restricted to COST-student)
Reaction clean up: beads purification
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08:00 - 09:00Epigenetics control in embryonic cell lines.
Dr. Susana Chuva Sousa Lopes. Dept of Anatomy and Embryology, Leiden University Medical Center
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09:00 - 10:00Epigenetics re-programming through the germ cell development and gametogenesis.
Dr. Susana Chuva Sousa Lopes. Dept of Anatomy and Embryology, Leiden University Medical Center
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10:00 - 12:00Impact of DNA methylation in human testicular tumors of germinal cells
Dr. Sebastian Cánovas. Dept of Physiology, University of Murcia
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13:30 - 16:30Lab session. Step V (restricted to COST-student)
Biotin capture, second strand synthesis and PCR amplification.
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07:00 - 08:30Lab session. Step VI (restricted to COST-student)
Library purification
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08:30 - 09:30Impact of epigenetics in embryonic development and adaptation
Dr. Alfonso Gutierrez Adan. Dept of Animal Reproduction INIA-Madrid
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10:30 - 12:00The theory of bisulphite methylation data processing and analysis
Dr. Simon Andrews. The Babraham Institute
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13:30 - 15:30Lab session. (restricted to COST-student)
Differential methylation analysis from bisulphite data.
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